SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 2. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Dagda Lab. The absorbance of formazan was detected at 540-nm wavelength with a reference wavelength at 650 nm for background using a SpectraMax M4 plate reader (Molecular Devices). Living cells were then incubated with 100 µl of medium assay provided by the assay kit. Choose from a three- (M3), four- (M4), orThe optical density was determined spectrophotometrically at 405 nm using a SpectraMax M4 microplate reader (Molecular Devices, Sunnyvale, CA). 1. For the microspheres containing moxifloxacin used in vivo, the liquid hydrogel was presoaked in a 24 mg/mL aqueous solution of moxifloxacin hydrochloride. 7°C and the bottom to 36. Background-subtracted data values (mean +/- SD) are graphed. D 1 1. Three kits covering a wide range of dsDNA concentrations. On day 2, in the morning weight the mice. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. However, traditional polyacrylamide gel electrophoresis (PAGE) and high. Full content visible, double tap to read brief content. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Lastly, the TMB substrate (Beyotime) was added, and absorbance was measured at 450 nm by a microplate reader (SpectraMax M4). Description 1. org) with optimized instrument settings (Table 1) were used to generate and analyze data. In the M2, M5 and Plus 384, the light. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e. To investigate the pharmacokinetics of HSA–CBT-NPs, 10 male Sprague-Dawley rats (mean±SD=250±20A SpectraMax M4 microplate reader was purchased from MeiGu Molecular Co. To report an issue with this product, Brief content visible, double tap to read full content. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeThen, cells were stained with MitoSox Red (5 μM) for 30 min. Louis, MO, USA). SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. In the competitive field of drug development where timely go/no-go decisions must be made to bring effective class-leading drugs to market, it is vital to work with a CRO that can provide highly. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. The BD plate dimensions are 5. Background-subtracted data values (mean +/- SD) are graphed. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. Polybead® nanoparticles were analyzed. To quantify F-CHP fluorescence, stained samples were digested in 500 µl of 1 mg/ml proteinase K in water for 3 hours at 60 °C. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate Reader The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The absorbance of solubilized formazan crystals was measured at 570 nm using a multimode reader (Spectramax M4, Molecular Devices LLC, Sunnyvale, CA, USA). ROS estimation by 2′,7′- dichlorofluorescin diacetate (DCFDA) staining . 4) In vivo pharmacokinetic studies. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. At sacrifice, serum was isolated from mouse total blood and maintained at –80 °C. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. com. Following digestion, 200 μl from each sample was pipetted into a 96-well plate, and samples were measured in duplicate at 485- and 525-nm excitation and emission, respectively (SpectraMax M4, Molecular Devices). 22 μM was mixed with 10-μl enzyme giving a final enzyme concentration in the. Large molecule bioanalysis, immunogenicity and PK testing with higher quality data, rapid turnaround and fewer surprises. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. Preconfigured SoftMax ® Pro Software protocols (available at softmaxpro. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forSpectraMax® M3. 05, ** p <. VIEW DETAILS The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 2. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Lower limits of detection for ATP determined using the ATPlite . com. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. 7. 2. Choose from a three- (M3), four- (M4), orThe absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). After incubation, the biofilms were washed with PBS until fully remove crystal violet solutions and then, solubilized by adding a 150 μL acetic acid. 2. The innovative and flexible design features. 7% of the total test aerosol mass) was not included in the collection analysis. AquaMax® 4000. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. ). The chloride and pH. 38. M2 M2e M3 M4 M5 M5e ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. We show that a ML model, trained on the. Size and zeta potential of nanoparticles were measured by Zetasizer Nano ZS90 (Malvern Instrument). (LMax II, SpectraMax L), with a Lower Limits of Detection (LLD) use six different light intensities for Detection Estimates, Magnitude 1 through 6. . . Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. S. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Ltd. C. The absorbance was determined by a microplate reader (SpectraMax M4, USA) at 405 nm. All luciferase assays were conducted in CORNING ® TypeI Collagen Coated 96-Well Cell Culture Clear Bottom White plates. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Table 2. Thirty min later, each well was homogenized and the optical density (OD) was measured in a spectrometer (Spectramax M4, Molecular Devices) at 570 nm. Results were expressed as the mean percentage of CV compared with NC group. e. Parameter SpectraMax iD5 SpectraMax i3x SpectraMax. Each well was scanned at nine evenly distributed points and averaged automatically with the software. 2. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). . The luciferase values were determined by normalization to Renilla values (as a control) to correct for variation in transfection efficiencies. 161 likes. Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) Total RNA was extracted at 48 h after exposure to volasertib, genistein, or gefitinib and quantified by a NanoDrop spectrophotometer (Thermo Scientific; Wilmington, DE, USA). Published Dec 16, 2020 | Updated Dec 16, 2020. D 47 7. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. The optical density was measured using a microplate reader (Spectramax M4, Molecular Devices, San Jose, CA, USA) at 570 nm. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. 1. Large Molecule Bioanalysis. The filtered samples were transferred to a 96-well plate and the fluorescence was measured using a plate reader (Spectramax ™ M4, Multi detection Reader) at excitation and emission wavelengths of λex 339 and λem 390 nm, respectively. ISM Equipment Classification (Group 1, Class A) This equipment is. Description 1. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable. 9 pg/mL. SpectraMax Microplate Readers. Diclofenac potassium at non-toxic concentration on U87-MG was used as positive control. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader MTT reduction - a tetrazolium-based colorimetric assay for cell survival and proliferation (Maxline Application Note #5) (PDF) Summary: The MTT assay measures cell respiration and the amount of formazan. Published Dec 16, 2020 | Updated Jan 25, 2021 Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax. The reaction systems were cultured in 37°C for 1 h, and the OD405 values were read on SpectraMax M4 (Molecular devices). 12. The absorbance was recorded using Spectramax M4 (Molecular Devices, USA) at 440 nm. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. 05 mol/L hydrochloric acid and 0. Sandwich ELISA - Human TNF alpha ELISA Kit, Fluorescent (ab229399) To learn more about the advantages of recombinant antibodies see here. High tolerance for contaminants. Italic and boldface type are used for emphasis. For more detailed information and technical support of Molecular Devices instruments or software,The sample solution was added to DPPH. . The medium was then removed and 100 μl of DMSO was added to dissolve the formazan crystal. 0 compensates for most changes in environmental temperature or pressure. to View Figures. Viability was determined by recording absorbance at 570 nm with plate reader (SpectraMax M4 Multimode, USA) and cell viability was determined. , 2020b). Fluoresbrite® fluorescent nanoparticles were analyzed by spectrofluorimetry where particles were excited at 441 nm wavelengths and emission intensity was recorded at 486 nm wavelengths. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. Phillips5, Martin A. At the end of the incubation period, MTT was added and the plates were incubated for 4 h at 37°C. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-Mode MicroplateReader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5e. The adherent cells were incubated in 100 μl of lysis buffer on ice for 20 min. Prepare 22 mg/mL of FD4, 16 mg/mL of rhodamine70, and 40 mg/mL of creatinine in sterile PBS. Optical density readings were obtained at various timepoints from 0 to 128 hours following inoculation using a SpectraMax M4 spectrophotometer (Molecular Devices, San Jose, CA). SpectraMax M4 Multi-Mode Microplate Reader. Expand the ABS1 Plate Inserted or Expt#3 experiment and select the OptAlign&UltDrk Plate section. 4. 2. D 1 1. AM (~0. The biofilm mass was quantified through the measurement of optical density of the sample at 570 nm using a spectrophotometer (SpectraMax® M4 Multi-Mode Microplate Reader, Molecular Devices). Choose from a three- (M3), four- (M4), or Qualify the luminescence performance of the SpectraMax . Eos - M4/3 lens adapter - Lenses - Victoria, British Columbia | Facebook. SpectraMax® Paradigm® Multi-Mode Detection Platform. Find M4 Group Marketing Specialists in Victoria, with phone, website, address, opening hours and contact info. Finally, the optical density was evaluated using SpectraMax M4 plate reader (Molecular Devices, San Jose, CA, USA) at 450 nm. Multi-Mode Microplate Readers. SpectraMax® M4. The Multi-mode readers are top and bottom read. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME-RESOLVED FLUORESCENCE PERFORMANCE SpectraMax M3 reader SpectraMax M4 reader SpectraMax M5 reader SpectraMax M5e reader Detection modes Absorbance ü ü ü ü Fluorescence intensity ü ü ü ü Luminescence ü ü ü ü Time-resolved fluorescence ü ü ü Fluorescence polarization ü ü HTRF ü Upgrade options TRF, HTRF, FP HTRF, FP HTRF N/A Plate formats The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SPECTRAMAX 190 reader: TRUE: Molecular Devices: SPECTRAMAX 340PC/340PC 384 reader: TRUE: Molecular Devices. Roelli1, Amandine Stoss1, Rebecca A. 05 mg mL −1. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Western blot apparatus (BIO-RAD Laboratories). The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. * BD Falcon® Microtest™ plates #353075 and # 353072). Mitochondrial respiration using high-resolution respirometry . Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates LM1, FlexStation 3 reader, SpectraMax L reader, LMax II (Obsolete), SpectraMax M5/M5e reader. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Figure 1. The fluorescence intensity was measured as ratio of values at an excitation at 340 nm and 380 nm with an emission at 510 nm, using Spectramax M4 (Molecular Devices). FD-4 concentrations in plasma were analyzed in duplicates using a spectrophotometer (SpectraMax M4, Molecular Devices, San Jose, CA, USA) with excitation λ 485 nm and emission λ 535 nm. Interpolated concentrations of native PF4 in human serum, plasma and cell culture supernatant samples. 3, La Jolla, CA, USA). 2. 5 mg mL −1 with 0. book Page 1 Wednesday, July 7, 2010 2:09 PM SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Absorbance was read at 570 nm with SpectraMax M4 microplate spectrophotometer (Molecular Devices, CA). Statistical. 7. . While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. There are multiple vendors. No. After 3 times washing, cells were added into 96-well plate and were examined under a fluorescence microplate reader (Excitation, 345 nm; Emission 495 nm) (SpectraMax M4 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA). 03 in x 3. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Luminescence was immediately detected by the SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA). SpectraMax M4 Multi-Mode Microplate Reader. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Cell viability was calculated using the formula as follows: (OD450 KO HepG2 -OD450 blank /OD450 WT HepG2 -OD450 blank ) × 100%. Click Read. Cet appareil ISM est confomre à la norme NMB-001 du Canada. New PBS 1X was added into each well and oxidized DCFH excitation and emission were measured at 485 and 525 nm respectively in a spectrophotometer (SpectraMax M4, Molecular Devices, Sunnyvale, USA). The DQ Green BSA probe. TSQ was dissolved in Lock’s buffer (pH 7. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. SpectraMax M Series Multi-Mode Microplate Readers | Molecular Devices · 2020-04-14 · use two scanning monochromators so the user can determine optimal excitation and emission settings, of 8 /8 Match case Limit results 1 per pageThe culture medium was removed, and the blue product was dissolved in DMSO (100 μl). Serum C-terminal telopeptide of type I collagen (S-CTX), osteocalcin (BGP), and tartrate-resistant acid phosphatase (TRAP) levels in serum and the receptor activator of the NF-κB ligand (RANKL), osteoprotegerin (OPG), and RANK levels in marrow were determinedThe ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. 05 mg mL −1. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. 2. The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. TSQ was dissolved in Lock’s buffer (pH 7. Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. The viability of cells was determined by using SpectraMax® M4 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA) at 570 nm absorbance . Detection modalities are shown in Table 1-1. 3, La Jolla, CA, USA). Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. These samples were analyzed using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Data analysis methods. qPCR The jejunum was sampled immediately after termination, snap frozen, andChoose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. When a feature or capability applies to only certain readers, this exception is noted. Category 2 and 3 microplate readers use gradient heating as well. 7°C (gradient heating design). STAKMAX. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. If using the StakMax and a SpectraMax reader together, the time specifications for the movement of the. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. , UK) or SpectraMax M4 by Molecular Device. The Dagda Lab is a mitochondrial biology-oriented research group located at the University of Nevada, Reno School of Medicine that currently spearheads basic and translational research projects supported. The IL-10 standard curve was prepared as described in Section 10. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politicsSpectraMax Spectrofluorometers. Molecular Devices SpectraMax® series microplate readers. 0. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. *Statistical significance: p < 0. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Moving a SpectraMax Multi-Mode Microplate Reader. After preparation, wrap in foil (see Note 1). The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. Description 1. At sacrifice, serum was isolated from mouse total blood and maintained at –80 °C. Turn the instrument around so that the back of the instrument is. The Insulin standard curve was prepared as described in Section 10. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Note: In this user guide, all references to SpectraMax Multi-Mode. Preconfigured SoftMax ® Pro Software protocols (available at softmaxpro. When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. All configurations. Table of Contents. SpectraMax ABS Plus SpectraTest ABS2 v1. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. 5 mg mL −1 with 0. Large molecule bioanalysis, immunogenicity and PK testing with higher quality data, rapid turnaround and fewer surprises. Plates were then read at 450 nm using a SpectraMax M4 device (Molecular Devices, Sunnyvale, CA). The combination index (CI) was calculated. Optimized for fluorescence microplate readers. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. 5 pM 2. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading 312 gain calibration failed. $2,219. The guide also covers the software features, the specifications, and the accessories of the systems. Microplate Readers include the M3, M4, exception is noted. Dumont3, Matthias S. 1. Table 2. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. The following document is intended to demonstrate setup of this instrument. SpectraMax® ABS. The percentage of viability of each treatment group in different. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. Analysis of flow cytometric samples was performed with BD FACSDiva software (BD Biosciences, San Jose, CA). normalized response (Graphpad Prism 8. The cleaved substrate was excited at 356-nm, and the fluorescence emissions were detected. All configurations. S. 95. The absorption value at 570 nm was measured using a Molecular Devices SpectraMax M4 plate reader, and the absorbance at 690 nm was subtracted as the background. D 47 7. SpectraMax® iD5. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. August 16, 2017. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. Background-subtracted data values (mean +/- SD) are graphed. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Examples: “Press carefully to engage,” “ Do not press down. Cell viability was calculated using the following formulaThe luciferase signal was read in a plate reader (SpectraMax M4; Molecular Devices, Sunnyvale, CA). For heme titration experiments a constant ATP concentration of 1 mM was used. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Plus 384 ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. The following document is intended to demonstrate setup of this instrument. StakMax Handling Stacker Base Unit. The drawing shows a standard microplate of 15 mm height covered with a lid. 1 M Tris solution and the fluorescence of the solution was measured using a fluorometer (SpectraMax M4, Molecular Devices). . The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. , and red fluorescence at 543 ex. All experiments were performed in triplicate. SpectraMax i3(x) SpectaTest ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. Five hours prior to when permeability will be measured, gavage mice with 0. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. Multiplate reader for ELISA detection (Spectramax M4). Note: In this user guide, all references to SpectraMax Multi-Mode. Molecular Devices LLC spectramax m4 multimode microplate reader page 12 25 Spectramax M4 Multimode Microplate Reader Page 12 25, supplied by Molecular Devices LLC, used in various techniques. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Images were taken using a fluorescence microscope (Nikon 80i, Japan). Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. All of the experiments were performed in triplicate, and the results were represented as mean. We therefore gathered salient information about many of the. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Measurements of temperature increase (ΔT) during the exposure of MNP/AMF hyperthermiaThe culture medium was removed, and the blue product was dissolved in DMSO (100 μl). for Cisbio Bioassays. Lift up the lid over the chamber to insert or remove a cuvette. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. When the user applies a set temperature of 37°C, the top is heated to 37. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. The optical absorbance of the plate at 450 nm was measured using a multifunctional microplate reader SpectraMax M4 (Molecular Devices, LLC, San Jose, CA, USA). For cell cultures, the medium was removed 24 h after initiation of drug stimulation and frozen for subsequent analysis, with no need for sample dilution. Dettmer4, Wayne A. MTT was then removed, and the formed formazan crystals were then dissolved in 150‐μl DMSO. Colony formation assays were performed to measure cell proliferation. 9000-0756. 次いで、ユーザーのラボに. N2a cells were induced for neurite growth by adding nerve growth factor (NGF) (50 ng/ml). 313 reference gain check fail. To determine the cell viability, thiazolyl blue tetrazolium bromide (MTT) assay was performed. After incubation, the fluorescence intensity was measured using Spectramax M4 (Molecular Devices) (excitation: 380 nm emission: 460 nm). Vol 4, Iss 22, Nov 20, 2014 . Cells were exposed to drug solutions with. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates LM1 , FlexStation 3. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Compare Fluorescence Microplate Readers from leading suppliers on Biocompare. The data represent the mean ± SD of three independent experiments. SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. 4 kilograms). 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Plate chamber design of the SpectraMax category 1 microplate readers. 1. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Then the formazan crystals were solubilized in dimethyl sulfoxide, and the absorbance was recorded at 572 nm wavelength using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. The medium was then removed and 100 μL of DMSO was added to dissolve the formazan crystal. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader 5 B > 4 4 5 B 5 : F 8 8 > 3 ; > I 5 = 8 5.